Difference between revisions of "Part:BBa K3696002"

 
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<partinfo>BBa_K3696002 short</partinfo>
 
<partinfo>BBa_K3696002 short</partinfo>
  
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BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings. The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;. From the detected fluorescence, we determine that HTDC system's detection limit for Pb2+ is 1 nM.
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<b>BBa_K3696000, BBa_K3696002--BBa_K3696003</b> are used in HTDC testings, the protocol is shown below:<br>
 +
[[File:T--OSA--protocol001.png|300px|thumb|center|HTDC protocol]]<br>
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<br>
 +
The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;.<br>
 +
[[File:T--OSA--part001.png|450px|thumb|center|HTDC Pb2+ testing]]<br>
 +
From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.<br>
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<br>
 +
<br>
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<b>BBa_K3696001, BBa_K3696002</b> are used in 8-17 DNAzyme testings, the protocol is shown below: <br>
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[[File:T--OSA--protocol002.png|600px|thumb|center|8-17 DNAzyme protocol]]<br>
 +
<br>
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The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;.<br>
 +
[[File:T--OSA--parts002.png|600px|thumb|center|8-17 DNAzyme(two parts) Pb2+ testing]]<br>
 +
From the detected fluorescence, we determine that: 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.<br>
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<br>
  
 
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===Usage and Biology===
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===Usage and Biology===-->
  
 
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Revision as of 17:31, 26 October 2020


The cleavable substrate of 8-17 DNAzyme.

BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings, the protocol is shown below:

HTDC protocol


The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

HTDC Pb2+ testing

From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.


BBa_K3696001, BBa_K3696002 are used in 8-17 DNAzyme testings, the protocol is shown below:

8-17 DNAzyme protocol


The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

8-17 DNAzyme(two parts) Pb2+ testing

From the detected fluorescence, we determine that: 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]