Difference between revisions of "Part:BBa K3424001:Design"
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=== Plasmid Design Notes=== | === Plasmid Design Notes=== | ||
| − | Make sure to check the | + | Make sure to check the source paper and type in the sequence of the terminator to see how the authors designed it. If you want to design another gene after this terminator in your plasmid, you should have around 19 intermediate bases before inserting a promoter for your other gene. |
| − | === | + | ===Source=== |
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3 | Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3 | ||
Revision as of 17:30, 26 October 2020
Plasmid Design Notes
Make sure to check the source paper and type in the sequence of the terminator to see how the authors designed it. If you want to design another gene after this terminator in your plasmid, you should have around 19 intermediate bases before inserting a promoter for your other gene.
Source
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3