Difference between revisions of "Part:BBa K3696002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | The actual sequence of 8-17 substrate strand is dCdAdGdTdTdAdCdAdTdTdCdT/i6FAM-dT/dCdTdAdTAdGdGdAdAdGdAdTdGdAdT/BHQ1 ("d" means the base after it is a DNA base). FAM is the fluorophore and BHQ1 is the fluorescence quencher. | ||
The FAM fluorophore is designed on the left of the cleavage site, while the BHQ1 fluorescence quencher is designed on the right of the cleavage site. This design allows the fluorophore to be separated from the fluorescence quencher once the substrate strand is cleaved. | The FAM fluorophore is designed on the left of the cleavage site, while the BHQ1 fluorescence quencher is designed on the right of the cleavage site. This design allows the fluorophore to be separated from the fluorescence quencher once the substrate strand is cleaved. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 17:30, 26 October 2020
The cleavable substrate of 8-17 DNAzyme.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The actual sequence of 8-17 substrate strand is dCdAdGdTdTdAdCdAdTdTdCdT/i6FAM-dT/dCdTdAdTAdGdGdAdAdGdAdTdGdAdT/BHQ1 ("d" means the base after it is a DNA base). FAM is the fluorophore and BHQ1 is the fluorescence quencher. The FAM fluorophore is designed on the left of the cleavage site, while the BHQ1 fluorescence quencher is designed on the right of the cleavage site. This design allows the fluorophore to be separated from the fluorescence quencher once the substrate strand is cleaved.
Source
The sequence of BBa_K3696002 is artificially designed. We adopted the sequence from the article "A hybridization-triggered DNAzyme cascade assay for enzyme-free amplified fluorescence detection of nucleic acid" and synthesized the part accordingly.