Difference between revisions of "Part:BBa K3463002"
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| − | + | Alginate lyase algL is an enzyme found in <i>Pseudomonas aeruginosa</i>. It is a 40 kDA poly-β-D-mannuronate lyase. AlgL degradates heteropolymer of β-D-mannuronate (M) and α-L-guluronate (G) that constitutes alginate. As alginate is the most abundant extracellular polysaccharide of the Pseudomonas aeruginosa PA01biofilm matrix, it has been shown that algL degradates PA01’s biofilm via β elimination. | |
| + | The gene coding for this enzyme has been cloned into pUCBB BBa_K3463000 and expressed in DH5 alpha Escherichia coli. | ||
| + | Source of the part: Genomic extraction by PCR on <i>Pseudomonas aeruginosa </i> PAO1. | ||
| + | Blanco-Cabra, N., Paetzold, B., Ferrar, T. et al. Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms. Sci Rep 10, 9390 (2020). | ||
| + | Design considerations: AlgL is regulated by AlgB in Pseudomonas aeruginosa genome. Should be added if production in <i>E. coli</i> is insufficient. Biosynthesis is higher when in presence of high salt concentrations. Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli.N L Schiller, S R Monday, C M Boyd, N T Keen, D E Ohman Journal of Bacteriology Aug 1993, 175 (15) 4780-4789; DOI:10.1128/jb.175.15.4780-4789.1993 | ||
| + | Compatible RFC10 | ||
Latest revision as of 17:14, 26 October 2020
Alginate lyase algL is an enzyme found in Pseudomonas aeruginosa. It is a 40 kDA poly-β-D-mannuronate lyase. AlgL degradates heteropolymer of β-D-mannuronate (M) and α-L-guluronate (G) that constitutes alginate. As alginate is the most abundant extracellular polysaccharide of the Pseudomonas aeruginosa PA01biofilm matrix, it has been shown that algL degradates PA01’s biofilm via β elimination. The gene coding for this enzyme has been cloned into pUCBB BBa_K3463000 and expressed in DH5 alpha Escherichia coli. Source of the part: Genomic extraction by PCR on Pseudomonas aeruginosa PAO1. Blanco-Cabra, N., Paetzold, B., Ferrar, T. et al. Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms. Sci Rep 10, 9390 (2020). Design considerations: AlgL is regulated by AlgB in Pseudomonas aeruginosa genome. Should be added if production in E. coli is insufficient. Biosynthesis is higher when in presence of high salt concentrations. Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli.N L Schiller, S R Monday, C M Boyd, N T Keen, D E Ohman Journal of Bacteriology Aug 1993, 175 (15) 4780-4789; DOI:10.1128/jb.175.15.4780-4789.1993 Compatible RFC10