Difference between revisions of "Part:BBa K3482036"

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(→‎Usage and Biology)
 
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===Usage and Biology===
 
===Usage and Biology===
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Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an l anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.
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Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.
  
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We used this part with a tag for protein purification or in a plasmid assembly for oscillatory expression
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We used this part with a tag for protein purification or in a repressilator plasmid for oscillatory expression.
  
 
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Latest revision as of 17:06, 26 October 2020


azurin with NSP4 secretion tag

Azurin protein fused with NSP4 secretion signial peptide

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.

We used this part with a tag for protein purification or in a repressilator plasmid for oscillatory expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]