Difference between revisions of "Part:BBa K3505026"
Line 5: | Line 5: | ||
long | long | ||
− | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | |||
− | |||
− | |||
+ | ===Design Notes=== | ||
+ | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for Golden Braid cloning. | ||
− | < | + | |
− | === | + | [[Image:T--Thessaly--B-ARRESTIN-TEV-PHOT.png|900px|thumb|none|<I><b>Figure 2.</b> The level B module of GPCR-Tango module : ParaBAD:RBS:B-arrestin -TEV protease -Double Terminator</i>]] |
− | <partinfo>BBa_K3505026 | + | |
− | + | ||
+ | ===Sequence and Features=== | ||
+ | <partinfo>BBa_K3505026 SequenceAndFeatures</partinfo> |
Revision as of 16:57, 26 October 2020
ParaBAD:RBS- β-arrestin2:TEVp:terminator
long
Usage and Biology
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector BBa_K3505009 and has overhangs compatible for Golden Braid cloning.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1446
Illegal BamHI site found at 1148
Illegal XhoI site found at 1843
Illegal XhoI site found at 2237 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2854
Illegal PstI site found at 1546
Illegal PstI site found at 1633
Illegal AgeI site found at 983
Illegal AgeI site found at 1829 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965
Illegal SapI.rc site found at 2788
Illegal SapI.rc site found at 3136