Difference between revisions of "Part:BBa K3467003:Experience"
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As the result shows: | As the result shows: | ||
| + | https://2020.igem.org/File:T--CAU_China--pw5.jpg | ||
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Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days. | Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days. | ||
| + | |||
| + | https://2020.igem.org/File:T--CAU_China--pw6.jpg | ||
Here is the survival rate that we measure during the whole experiment process. | Here is the survival rate that we measure during the whole experiment process. | ||
Revision as of 16:53, 26 October 2020
In our project, we designed three basic part, targeting to three different genes of locust. In our design, we selected Locust migratoria gene trypsin-like serine protease, chitin synthase and ATPase subunitH gene as our target gene. By using specific program to design dsRNA sequence, we finally designed our three basic parts: TSP dsRNA sequence, CHS dsRNA sequence and ATPase dsRNA sequence.
We also measured their effect to locust by injection experiment.
As the result shows:
https://2020.igem.org/File:T--CAU_China--pw5.jpg
Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days.
https://2020.igem.org/File:T--CAU_China--pw6.jpg
Here is the survival rate that we measure during the whole experiment process.