Difference between revisions of "Part:BBa K3505025"
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for Golden Braid cloning. | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for Golden Braid cloning. | ||
− | [[Image:T--Thessaly--FFAR2-VTAIL-PHOT.png|900px|thumb|none|<I><b>Figure 2.</b> The level B module of GPCR-Tango Module : a1R:ParaBAD:RBS-FFAR2:V2tail:TCS- | + | [[Image:T--Thessaly--FFAR2-VTAIL-PHOT.png|900px|thumb|none|<I><b>Figure 2.</b> The level B module of GPCR-Tango Module : a1R:ParaBAD:RBS-FFAR2:V2tail:TCS-Lac-Double terminator </i>]] |
===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K3505025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3505025 SequenceAndFeatures</partinfo> |
Revision as of 16:37, 26 October 2020
ParaBAD:RBS-FFAR2:AVPR2 tail:TCS-LacI-terminator
long
Usage and Biology
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector BBa_K3505008 and has overhangs compatible for Golden Braid cloning.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1527
Illegal PstI site found at 2107 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1527
Illegal PstI site found at 2107 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1148
Illegal BamHI site found at 1349 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1527
Illegal PstI site found at 2107 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1527
Illegal PstI site found at 2107
Illegal NgoMIV site found at 1607
Illegal AgeI site found at 983 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965
Illegal SapI site found at 1803
Illegal SapI site found at 2058
Illegal SapI.rc site found at 1260