Difference between revisions of "Part:BBa K133011"

Revision as of 21:18, 29 October 2008

CF213-UreB-linker

CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215) In combination with CF215 it forms a TLR5 activating protein.

UreB – beta subunit of H. pylori urease that we used as an antigen

Linker enables easier and more correct folding of the protein.

This part is an intermediate to [1].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1655
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 301
Illegal AgeI site found at 877
Illegal AgeI site found at 2055
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 763

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K133011 short</partinfo>
-CF213-UreB-LINKER
+CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal  portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
+In combination with CF215 it forms a TLR5 activating protein.
+UreB – beta subunit of H. pylori urease that we used as an antigen
+Linker enables easier and more correct folding of the protein.
+This part is an intermediate to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K133012].