Difference between revisions of "Part:BBa K3463011:Experience"

 
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K3463011===
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===Usage and Biology BBa_K3463011===
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The Figure 1 illustrates the fluorescence intensity over time for 2 growing cultures of <i>E. coli </i> Nissle transformed with the plasmid pUCBB PrhlR-eGFP BBa_K3463011. Cultures were performed in absence or in presence of 100µM of synthetic BHL.
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In addition, a culture of wild type <i>E. coli </i> Nissle (not transformed) was used as a negative control.
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The fluorescence intensity was monitored for 15 hours. Each time point was performed hourly in triplicata. For each curve, the first measurement (T0) was set at 0 and subtracted to following ones.
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This experiment was repeated 3 times.
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[[Image:T--Grenoble Alpes--Res Survival fluo prhlR.png|center| '''Figure 1'''  eGFP expression according to the BHL and over time. Two conditions with 100µM of BHL and without BHL were performed to evaluate the effect of BHL in E. coli Nissle. With and without BHL, no eGFP expression should be detected because there is no production of RhlR protein.]]
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===User Reviews===
 
===User Reviews===

Revision as of 15:34, 26 October 2020


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Usage and Biology BBa_K3463011

The Figure 1 illustrates the fluorescence intensity over time for 2 growing cultures of E. coli Nissle transformed with the plasmid pUCBB PrhlR-eGFP BBa_K3463011. Cultures were performed in absence or in presence of 100µM of synthetic BHL. In addition, a culture of wild type E. coli Nissle (not transformed) was used as a negative control.

The fluorescence intensity was monitored for 15 hours. Each time point was performed hourly in triplicata. For each curve, the first measurement (T0) was set at 0 and subtracted to following ones. This experiment was repeated 3 times.

Figure 1 eGFP expression according to the BHL and over time. Two conditions with 100µM of BHL and without BHL were performed to evaluate the effect of BHL in E. coli Nissle. With and without BHL, no eGFP expression should be detected because there is no production of RhlR protein.




User Reviews

UNIQaa239fb170a2dfae-partinfo-00000000-QINU UNIQaa239fb170a2dfae-partinfo-00000001-QINU