Difference between revisions of "Part:BBa K3693010:Design"
This-is-Min (Talk | contribs) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | We add his-tag at both N-terminal and C-terminal for affinity purification | ||
===Source=== | ===Source=== | ||
| − | + | The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization. | |
===References=== | ===References=== | ||
Latest revision as of 15:22, 26 October 2020
Purification of CSN3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add his-tag at both N-terminal and C-terminal for affinity purification
Source
The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization.