Difference between revisions of "Part:BBa K3693009:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | We add his-tag at both N-terminal and C-terminal for affinity purification | ||
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===Source=== | ===Source=== | ||
| − | + | The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization. | |
===References=== | ===References=== | ||
Latest revision as of 15:17, 26 October 2020
Purification of CSN2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add his-tag at both N-terminal and C-terminal for affinity purification
Source
The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization.