Difference between revisions of "Part:BBa K3693008:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Affinity purification  
+
 
 +
We add his-tag at both N-terminal and C-terminal for affinity purification  
  
  
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===Source===
 
===Source===
  
−
It is synthetic
+
The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization.
  
 
===References===
 
===References===

Latest revision as of 15:16, 26 October 2020


Purification of LTF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
    Illegal BglII site found at 859
    Illegal BglII site found at 1414
    Illegal BglII site found at 1585
    Illegal BglII site found at 1846
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 309
    Illegal BsaI.rc site found at 483
    Illegal SapI.rc site found at 1109


Design Notes

We add his-tag at both N-terminal and C-terminal for affinity purification


Source

The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization.

References