Difference between revisions of "Part:BBa K3693004:Design"
 
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===Design Notes===
 
===Design Notes===
−
It should be capable to stimulation the expression of kappa-casein.  
+
 
 +
We add a his tag between secretion signal and gene for purification.
  
  
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===Source===
 
===Source===
  
−
It is synthetic.
+
The alpha-secretion signal is from pichia pastoris. His-tag is we designed and added artificially. The original sequence of CSN3 is from Homo sapiens but we did codon optimization.
  
 
===References===
 
===References===

Latest revision as of 14:52, 26 October 2020


For secreting the κ-casein outside yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a his tag between secretion signal and gene for purification.


Source

The alpha-secretion signal is from pichia pastoris. His-tag is we designed and added artificially. The original sequence of CSN3 is from Homo sapiens but we did codon optimization.

References