Difference between revisions of "Part:BBa K3505016"

Revision as of 14:49, 26 October 2020

araC-ParaBAD:RBS GB compatible with A1-B1

long

Usage and Biology

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. The CDS has position A1-B2

Figure 1.The overhangs of this part in the Golden Braid Grammar.

Figure 2. The level 0 module : pupd2- araC:ParaBAD (illustration from SnapGene)

Verification of Cloning

Fig.1:(U=Uncut C=Cut) Restriction Digestion of araC:ParaBAD with EcoRI+ PstI ,Expected bands: 2029+1996, Positive result : YES

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1148
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 983
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 965

@@ Line 5: / Line 5: @@
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-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+===Design Notes===
-<span class='h3bb'>Sequence and Features</span>
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 <b>BBa_K3505007 </b> and has overhangs compatible for Golden Braid cloning.
+The CDS has position A1-B2
+<p style="text-align: center;">
+    [[Image:T--Thessaly--GB-GGAG-CCAT.jpeg |900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the Golden Braid Grammar.</i>]]
+ [[Image:T--Thessaly--paracBAD-snap.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- araC:ParaBAD (illustration from SnapGene)</i>]]
+===Verification of Cloning===
+[[File:T--Thessaly--araBAD-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction Digestion of araC:ParaBAD with EcoRI+ PstI ,Expected bands: 2029+1996, Positive result : YES </i>]]
+===Sequence and Features===
 <partinfo>BBa_K3505016 SequenceAndFeatures</partinfo>