Difference between revisions of "Part:BBa K3693004"
 
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<partinfo>BBa_K3693004 short</partinfo>
 
<partinfo>BBa_K3693004 short</partinfo>
  
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The composite part consist the a secretion signal E.coli that can trigger the expression of the protein exerted in, and the sequence of CSN3 we exerted in. This sequence enables the bacteria produce kappa-casein.
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The composite part consists of a secretion signal for pichia pastoris that can trigger the expression of the protein exerted in, and followed by a his tag for purification, then the sequence of CSN3 we exerted in. This sequence enables the yeast to produce kappa-casein and secrete it outside.
  
 
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Latest revision as of 14:48, 26 October 2020


For secreting the κ-casein outside yeast

The composite part consists of a secretion signal for pichia pastoris that can trigger the expression of the protein exerted in, and followed by a his tag for purification, then the sequence of CSN3 we exerted in. This sequence enables the yeast to produce kappa-casein and secrete it outside.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]