Difference between revisions of "Part:BBa K3525000"

 
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BBa_K3525000 is a sensor, used to detect endocrine-disrupting chemicals (EDCs).
 
BBa_K3525000 is a sensor, used to detect endocrine-disrupting chemicals (EDCs).
  
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===Usage and Biology===
 
  
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===Contribution===
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<span class='h3bb'>Sequence and Features</span>
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Compared to the existing part BBa_I732005 which is a full-length lacZ, we insert hERα and intein into the lacZ to produce a new part BBa_K3525000, giving it a new function of detecting the presence of estrogenic chemicals in environmental water.
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<partinfo>BBa_K3525000 SequenceAndFeatures</partinfo>
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[[File:T--Shanghai high school-BBa K3525000 fig 0.png|500px|thumb|center|figure 1]]
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We obtained a chimeric protein composed of S. cerevisiae VMA intein, the esterogen binding domain of hERα and lacZ reporter protein. This protein may provide a complete and independent biosensor for estrogen molecular. In theory, estrogen dependent self splicing and production of active lacZ protein can be realized by this chimeric protein. The related domain parts and design principles may also be used for sensor proteins of other molecules.
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===Functional Parameters===
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<partinfo>BBa_K3525000 parameters</partinfo>
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Revision as of 14:44, 26 October 2020


ER sensor

BBa_K3525000 is a sensor, used to detect endocrine-disrupting chemicals (EDCs).


Contribution

Compared to the existing part BBa_I732005 which is a full-length lacZ, we insert hERα and intein into the lacZ to produce a new part BBa_K3525000, giving it a new function of detecting the presence of estrogenic chemicals in environmental water.

figure 1

We obtained a chimeric protein composed of S. cerevisiae VMA intein, the esterogen binding domain of hERα and lacZ reporter protein. This protein may provide a complete and independent biosensor for estrogen molecular. In theory, estrogen dependent self splicing and production of active lacZ protein can be realized by this chimeric protein. The related domain parts and design principles may also be used for sensor proteins of other molecules.