Difference between revisions of "Part:BBa K157041:Design"
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===References=== | ===References=== | ||
| + | - W. Bruening, B. Giasson, W. Mushynski1 and H. D. Durham (1998): Activation of stress-activated MAP protein kinases up-regulates expression of transgenes driven by the cytomegalovirus immediate/early promoter, in Nucleic Acids Research, 1998, Vol. 26, No. 2 | ||
Latest revision as of 21:10, 29 October 2008
Transfection vector (similar to pSB1AK8)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Illegal EcoRI site found at 3405
Illegal XbaI site found at 3420 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3411 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XhoI site found at 1272
Illegal XhoI site found at 2298 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found at 2
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3405
Plasmid lacks a suffix.
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2444
Design Notes
CMV Promotor is a viral regulatory element to mediate strong expression of the downstream gene sequence. CMV stands for cytomegalovirus. Because of its strong activity in most cells its a very popular choice to mediate expression of a foreign protein.
Source
Derived by cloning of the parts BBa_K157040 and BBa_I757009.
References
- W. Bruening, B. Giasson, W. Mushynski1 and H. D. Durham (1998): Activation of stress-activated MAP protein kinases up-regulates expression of transgenes driven by the cytomegalovirus immediate/early promoter, in Nucleic Acids Research, 1998, Vol. 26, No. 2