Difference between revisions of "Part:BBa K3505034"
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<partinfo>BBa_K3505034 short</partinfo> | <partinfo>BBa_K3505034 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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− | < | + | ===Design Notes=== |
+ | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha vector <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for Golden Braid cloning. | ||
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+ | [[Image:T--Thessaly--teO-LacI-PHOT.png|900px|thumb|none|<I><b>Figure 2.</b> The level A module of Tet-Off system : AndersonJ23115:TetO:RBS-LacI-Double terminator </i>]] | ||
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+ | ===Verification of Cloning=== | ||
+ | [[File:T--Thessaly--tetO-LacI-digestionpng.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction digestion of TL: AndersonJ23115:Tet0-LacI-double terminator(C5-C8 ) with: HindIII + NheI (C5-C18) , Expected bands : 2573+891+427,Positive result : C5,C6</i>]] | ||
+ | ===Sequence and Features=== | ||
<partinfo>BBa_K3505034 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3505034 SequenceAndFeatures</partinfo> | ||
Revision as of 14:18, 26 October 2020
pAndersonJ23115:tetO:RBS-lacI-terminator
Usage and Biology
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha vector BBa_K3505009 and has overhangs compatible for Golden Braid cloning.
Verification of Cloning
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]