Difference between revisions of "Part:BBa K157040"

Revision as of 21:00, 29 October 2008

Eukaryotic terminator vector (very similar to pSB1AK8)

Transfection vector with CMV Promotor for expression in eucaryotic cells. This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 8
Illegal NotI site found at 3411
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XhoI site found at 1272
Illegal XhoI site found at 2298
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 16
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3405
Plasmid lacks a suffix.
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2444

@@ Line 3: / Line 3: @@
 Transfection vector with CMV Promotor for expression in eucaryotic cells.
+This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
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