Difference between revisions of "Part:BBa K190021"

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SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.  
 
SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.  
 
+
<center>https://2020.igem.org/File:T--CSU_CHINA--Contribution_SmtA.png</center><br>
  
 
===Usage and Biology===
 
===Usage and Biology===
 
The gene was functionally expressed in ''E. coli''.
 
The gene was functionally expressed in ''E. coli''.
 
The protein can be used to accumulate Zn(II) intracellular.  
 
The protein can be used to accumulate Zn(II) intracellular.  
 +
 +
===Supplementary Experiment===
 +
Group: iGEM20_CSU_CHINA (2020-10-26)
 +
Author: Hao Zhou
 +
Summary:
 +
  
 
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Revision as of 13:02, 26 October 2020

SmtA

SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.

https://2020.igem.org/File:T--CSU_CHINA--Contribution_SmtA.png

Usage and Biology

The gene was functionally expressed in E. coli. The protein can be used to accumulate Zn(II) intracellular.

Supplementary Experiment

Group: iGEM20_CSU_CHINA (2020-10-26) Author: Hao Zhou Summary:


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal AgeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]