Difference between revisions of "Part:BBa K3396006"

Revision as of 12:41, 26 October 2020

GFPnano-Coh2

To make sure that GFPnano-GFP can connect with TRIM21-DocS, we designed the part which bind Coh2 and GFPnano.

Usage and Biology

Coh2 is a kind of protein which bind with DocS spontaneously. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part can target the protein and connect with TRIM21-DocS and lead to the degradation in a Trim-Away alike method. Here is the mechanism of the recombined TRIM21-DocS:

1. The GFPnano tagged with Coh2 combines with targeted protein.

2. TRIM21-DocS connect Coh2-GFPnano-target through the DocS-Coh2 interaction.

3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 35

@@ Line 5: / Line 5: @@
 To make sure that GFPnano-GFP can connect with TRIM21-DocS, we designed the part which bind Coh2 and GFPnano.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+Coh2 is a kind of protein which bind with DocS spontaneously. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part can target the protein and connect with TRIM21-DocS and lead to the degradation in a Trim-Away alike method. Here is the mechanism of the recombined TRIM21-DocS:
+.	The GFPnano tagged with Coh2 combines with targeted protein.
+.	TRIM21-DocS connect Coh2-GFPnano-target through the DocS-Coh2 interaction.
+.	The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3396006 SequenceAndFeatures</partinfo>