Difference between revisions of "Part:BBa K3365054:Experience"
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We add this part into off-target detection platform(part BBa_K3365062, BBa_K3365063, BBa_K3365064 and BBa_K3365065) as a reporter in the following three steps: | We add this part into off-target detection platform(part BBa_K3365062, BBa_K3365063, BBa_K3365064 and BBa_K3365065) as a reporter in the following three steps: | ||
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Firstly, we add target sequence to the head of spacer by conducting PCR-primer used in this procedure contains the target sequence. | Firstly, we add target sequence to the head of spacer by conducting PCR-primer used in this procedure contains the target sequence. | ||
Secondly, we use Overlap PCR to connect these two fragments, for fear that target-blank fragment is too short to be attached to the eGFP sequence. | Secondly, we use Overlap PCR to connect these two fragments, for fear that target-blank fragment is too short to be attached to the eGFP sequence. | ||
| − | Thirdly, we obtain terminator by performing PCR on pE1a-RFP and then link target+blank+eGFP and terminator through overlap PCR. | + | Thirdly, we obtain terminator by performing PCR on pE1a-RFP and then link target+blank+eGFP and terminator through overlap PCR. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:17, 26 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3365054
We add this part into off-target detection platform(part BBa_K3365062, BBa_K3365063, BBa_K3365064 and BBa_K3365065) as a reporter in the following three steps:
Firstly, we add target sequence to the head of spacer by conducting PCR-primer used in this procedure contains the target sequence. Secondly, we use Overlap PCR to connect these two fragments, for fear that target-blank fragment is too short to be attached to the eGFP sequence. Thirdly, we obtain terminator by performing PCR on pE1a-RFP and then link target+blank+eGFP and terminator through overlap PCR.
User Reviews
UNIQ9dc21b8661820402-partinfo-00000000-QINU UNIQ9dc21b8661820402-partinfo-00000001-QINU