Difference between revisions of "Part:BBa K3396002"

 
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Proving that RING domain on TRIM21 can still conduce degradation on protein through recruiting ubiquitin-proteasome after replacing its PRYSPRY with DocS, we replace the DocS with FRB.
 
Proving that RING domain on TRIM21 can still conduce degradation on protein through recruiting ubiquitin-proteasome after replacing its PRYSPRY with DocS, we replace the DocS with FRB.
  
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===Usage and Biology===
 
===Usage and Biology===
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FRB is a kind of protein which can bind with FKBP induced by rapamycin. TRIM21 is an E3 ubiquitin-protein ligase which lead to degradation. To demonstrate that the Trim-Away alike method can be performed after replacing the DocS-Coh2 with FRB-FKBP interaction, we designed TRIM21-FRB. Here is the mechanism of the recombined TRIM21-FRB:
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1. The GFPnano tagged with FRB combines with targeted protein.
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2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction.
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The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3396002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3396002 SequenceAndFeatures</partinfo>

Revision as of 12:14, 26 October 2020


TRIM21-FRB

Proving that RING domain on TRIM21 can still conduce degradation on protein through recruiting ubiquitin-proteasome after replacing its PRYSPRY with DocS, we replace the DocS with FRB.

Usage and Biology

FRB is a kind of protein which can bind with FKBP induced by rapamycin. TRIM21 is an E3 ubiquitin-protein ligase which lead to degradation. To demonstrate that the Trim-Away alike method can be performed after replacing the DocS-Coh2 with FRB-FKBP interaction, we designed TRIM21-FRB. Here is the mechanism of the recombined TRIM21-FRB: 1. The GFPnano tagged with FRB combines with targeted protein. 2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1088
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]