Difference between revisions of "Part:BBa K3338002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-κB/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. It was designed as an improvement of the CMV promoter making it LPS-sensing. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated AP1-NF-κB binding sites are localized. | + | The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-κB/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. It was designed as an improvement of the CMV promoter making it LPS-sensing. This improvment is also documented on the wiki page of the original part <html><a href="https://parts.igem.org/Part:BBa_I712004">BBa_I712004</a></html>. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated AP1-NF-κB binding sites are localized. |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 11:51, 26 October 2020
Synthetic promoter_2 with NF-κB and AP1 binding sites
Usage and Biology
The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-κB/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. It was designed as an improvement of the CMV promoter making it LPS-sensing. This improvment is also documented on the wiki page of the original part BBa_I712004. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated AP1-NF-κB binding sites are localized.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
Illegal NotI site found at 58 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]