Difference between revisions of "Part:BBa K3407006:Design"
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<partinfo>BBa_K3407006 short</partinfo> | <partinfo>BBa_K3407006 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | Design | + | This biobrick represents the DNA template for the transcription of a 65 nt shRNA. The dsRNA sequence was taken from pUC57-OriLR-deGFP plasmid (BBa_K3407006). It corresponds to 27nt from the eGFP gene (nt 78 to 105), and is the one recognised and processed by Dicer to form siRNA. The shRNA has a single-stranded RNA (ssRNA) loop region designed to be recognised by Fox-1 RBD (<html><a id="6" href="https://parts.igem.org/Part:BBa_K3407004" target="_blank"><b>BBa_K3407004</b></a><html>), containing its “<html><u>UGCAUGU</u><html>” RNA target sequence. The dsRNA region contains a GG overhang that would represent the overhang left when Mini-3 (BBa_K3407002) cleaves a tshRNA (See iGEM TU Delft 2020, Design - Future perspectives), and that represents a good substrate to process the shRNA by Dicer into a siRNA. |
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Revision as of 11:48, 26 October 2020
Short hairpin RNA (shRNA): a dmDicer-2 substrate and potential trigger of RNAi.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This biobrick represents the DNA template for the transcription of a 65 nt shRNA. The dsRNA sequence was taken from pUC57-OriLR-deGFP plasmid (BBa_K3407006). It corresponds to 27nt from the eGFP gene (nt 78 to 105), and is the one recognised and processed by Dicer to form siRNA. The shRNA has a single-stranded RNA (ssRNA) loop region designed to be recognised by Fox-1 RBD (BBa_K3407004), containing its “UGCAUGU” RNA target sequence. The dsRNA region contains a GG overhang that would represent the overhang left when Mini-3 (BBa_K3407002) cleaves a tshRNA (See iGEM TU Delft 2020, Design - Future perspectives), and that represents a good substrate to process the shRNA by Dicer into a siRNA. ===Source=== Source here ===References===