Difference between revisions of "Part:BBa K3454037"
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This part is the template for AsCas12a extraction and purfication for reaction system. It consists of three parts, which are AsCas12a coding sequence, MBP tag and His tag. | This part is the template for AsCas12a extraction and purfication for reaction system. It consists of three parts, which are AsCas12a coding sequence, MBP tag and His tag. | ||
+ | |||
+ | ===Characterization=== | ||
+ | ====Results==== | ||
+ | We induced AsCas12a expression by IPTG and purified it by gravity-flow column with Ni-NTA Sefinose(TM) Resin. CBB(Coomassie brilliant blue) is used to characterize protein purification as shown in Figure 1. | ||
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Revision as of 11:30, 26 October 2020
AsCas12a
This part is the template for AsCas12a extraction and purfication for reaction system. It consists of three parts, which are AsCas12a coding sequence, MBP tag and His tag.
Characterization
Results
We induced AsCas12a expression by IPTG and purified it by gravity-flow column with Ni-NTA Sefinose(TM) Resin. CBB(Coomassie brilliant blue) is used to characterize protein purification as shown in Figure 1.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 886
Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3855
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865
Illegal NgoMIV site found at 3399
Illegal AgeI site found at 2296
Illegal AgeI site found at 2767 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1039
Illegal BsaI.rc site found at 2701
Illegal SapI.rc site found at 289