Difference between revisions of "Part:BBa K3505005"

 
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===Usage and Biology===
 
===Usage and Biology===
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<p style="text-align: center;">
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    [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the Golden Braid Grammar.</i>]]
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[[Image:T--Thessaly--tetR-snap.png |900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- TetR (illustration from SnapGene)</i>]]
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===Verification of Cloning===
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[[File:T--Thessaly--tetr-level0-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with :
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SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp
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Positive result : C4 �</i>]]
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Revision as of 10:27, 26 October 2020


TetR- Tet repressor GB compatible with B3-B5

TetR- tetR

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]