Difference between revisions of "Part:BBa K3521001"
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[[File:T--SHSID-BBa_K3521001 Fig 0.jpg|400px|thumb|center|Cpf1]] | [[File:T--SHSID-BBa_K3521001 Fig 0.jpg|400px|thumb|center|Cpf1]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Our new part is used to detect the presence of Listeria monocytogenes, which is easily survival in low temperature like refrigerator environment,and then cause Listeriosis illness, like fever, muscle ache, and so on. | Our new part is used to detect the presence of Listeria monocytogenes, which is easily survival in low temperature like refrigerator environment,and then cause Listeriosis illness, like fever, muscle ache, and so on. | ||
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| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K3521001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3521001 SequenceAndFeatures</partinfo> | ||
| − | + | ===Engineering Success=== | |
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Revision as of 08:51, 26 October 2020
FnCpf1
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) systems are adaptive immune systems widely distributed across bacteria and archaea, designed to destroy DNA of invading mobile genetic elements. These immune systems, in which endonucleases are guided by single stranded RNA to find their target DNA, have been turned into powerful genome editing tools over the past few years FnCpf1 is one of class II family of CRISPR-Cas RNA programmable endonucleases with unique functions. In this project, FnCpf1 can mediate RNA-guided DNA cleavage and target single-stranded DNA to produce fluorescence.
Usage and Biology
Our new part is used to detect the presence of Listeria monocytogenes, which is easily survival in low temperature like refrigerator environment,and then cause Listeriosis illness, like fever, muscle ache, and so on.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1717
Illegal NheI site found at 3437 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3498
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]