Difference between revisions of "Part:BBa K3696008:Design"

 
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===Design Notes===
 
===Design Notes===
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As part of CRISPR-Cas12a system, we adopted the sequence of BBa_K3696008 (crRNA) from other articles. Therefore it is essential to be careful with other parts that will bind with crRNA in Cas12a reaction. For instance, ssDNA with different length has different effect on the reaction.
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As part of CRISPR-Cas12a system, we adopted the sequence of BBa_K3696008 (crRNA) from other articles. Therefore it is essential to be careful with ssDNA that will bind with crRNA in Cas12a reaction, because these ssDNA may be affected by the design of upstream signal sources.
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===Source===
 
===Source===

Revision as of 08:42, 26 October 2020


A guide RNA of CRISPR-Cas12a system which can bind to target DNA and Cas12a nuclease.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As part of CRISPR-Cas12a system, we adopted the sequence of BBa_K3696008 (crRNA) from other articles. Therefore it is essential to be careful with ssDNA that will bind with crRNA in Cas12a reaction, because these ssDNA may be affected by the design of upstream signal sources.

Source

The sequence of BBa_K3696008 is artificially designed. We adopted the sequence from the article "CRISPR-Cas12a has both cis- and trans- cleavage activities on single-stranded DNA" and synthesized the part accordingly.

References