Difference between revisions of "Part:BBa K3582020"

Latest revision as of 07:41, 26 October 2020

Modified C-intein piece

C and N inteins are widely used in the circularizing mechanism of proteins. We plan to use the c-intein to build a composite construction that will help us circularize the protein that we insert within the C and the N inteins. This biobrick is designed by modifying the biobrick BBa_K1362401 designed by the Heidelberg 2014 team(since it was out of the frame and incompatible with RF-cloning). We have modified it to be in frame with the construct making it suitable for performing RF cloning/Gibson's Assembly.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3582020 short</partinfo>
-Inteins can be found in all kingdoms of life, however, their use for the organism remains yet unknown. We plan to use the c-intein to build a composite construction that will help us circularize the protein that we insert within the C and the N inteins. This biobrick is designed by modifying the biobrick BBa_K1362401 designed by the Heidelberg 2014 team. We have modified it to be in frame with the construct making it suitable for performing Gibson's Assembly.
+C and N inteins are widely used in the circularizing mechanism of proteins. We plan to use the c-intein to build a composite construction that will help us circularize the protein that we insert within the C and the N inteins. This biobrick is designed by modifying the biobrick BBa_K1362401 designed by the Heidelberg 2014 team(since it was out of the frame and incompatible with RF-cloning). We have modified it to be in frame with the construct making it suitable for performing RF cloning/Gibson's Assembly.
 <!-- Add more about the biology of this part here