Difference between revisions of "Part:BBa K3582023"
 
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<partinfo>BBa_K3582023 short</partinfo>
 
<partinfo>BBa_K3582023 short</partinfo>
  
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The biobrick codes for cyclotide kalata B1 protein domain consisting of first 5 loops of the cyclotide construct.
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The biobrick codes for the cyclotide kalata B1 protein domain consisting of the first 5 loops of the cyclotide construct.
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This part is created to allow insertion of peptide sequences in the 6th loop.
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This part has been created to allow insertion of peptide sequences in the 6th loop.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:33, 26 October 2020


Cyclotide Kalata B1 domain with strep II tag

The biobrick codes for the cyclotide kalata B1 protein domain consisting of the first 5 loops of the cyclotide construct. This part has been created to allow insertion of peptide sequences in the 6th loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 29

References

  • 1] Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery. (2011, December 1). PubMed

Central (PMC). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330703/

  • 2] Poth, A. G. (2013, May 7). Cyclotides as Grafting Frameworks for Protein Engineering and Drug Design

Applications. PubMed. https://pubmed.ncbi.nlm.nih.gov/23893608/

  • 3] 2. Camarero, J. A. (2019, April 19). The Potential of the Cyclotide Scaffold for Drug Development.

MDPI. https://www.mdpi.com/2227-9059/7/2/31/htm#B31-biomedicines-07-00031