Difference between revisions of "Part:BBa K3447011"

 
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P<sub>2</sub> promoter is an inducible promoter that activates downstream gene expression after phosphorylated AgrA protein dimerization and binding to it.
 
P<sub>2</sub> promoter is an inducible promoter that activates downstream gene expression after phosphorylated AgrA protein dimerization and binding to it.
  
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===Usage and Biology===
 
===Usage and Biology===
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The expression of virulence factors in Staphylococcus aureus is strictly controlled by an auto-induced quorum sensing system called the auxiliary gene Regulator (Agr) system. The agrD gene encodes the signaling propeptide, and the agrB gene encodes the peptidase used to process the signaling propeptide. AgrD mature peptide is the signal peptide of Agr system, also known as self-induced peptide (AIP) molecule. AgrC and AgrA constitute a two-part system. AgrC is the receptor for AIP. Upon binding to AIP, AgrC dimerizes and phosphorylates each other to activate AgrC proteins. AgrC can phosphorylate AgrA protein. The phosphorylated AgrA protein dimerizes and binds to the P2 promoter of the agrBDCA operon to form a positive feedback loop. The activity of AgrA dimers will also combine with P3 promoter, to activate the non-coding RNA, the RNA III expression. RNA III can affect the virulence factor and adhesion factor of the translation process.<br>
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[[Image: Diagram of the Agr system of Staphylococcus aureus.jpg|center|frame|100px|<b>Figure 1. </b> Diagram of the Agr system of Staphylococcus aureus]]<br><br>
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We verified the expression capability by connecting sfGFP gene downstream (链接).
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==<b>Design</b>==
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===Design Notes===
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We added some synonymous mutations to avoid part rules.
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===Source===
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We found this sequence data in the reported EMBL/GenBank/DDBJ Nucleotide Sequence Database.
  
 
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Revision as of 06:31, 26 October 2020


P2, the inducible type turns on the expression of downstream genes

P2 promoter is an inducible promoter that activates downstream gene expression after phosphorylated AgrA protein dimerization and binding to it.

Usage and Biology

The expression of virulence factors in Staphylococcus aureus is strictly controlled by an auto-induced quorum sensing system called the auxiliary gene Regulator (Agr) system. The agrD gene encodes the signaling propeptide, and the agrB gene encodes the peptidase used to process the signaling propeptide. AgrD mature peptide is the signal peptide of Agr system, also known as self-induced peptide (AIP) molecule. AgrC and AgrA constitute a two-part system. AgrC is the receptor for AIP. Upon binding to AIP, AgrC dimerizes and phosphorylates each other to activate AgrC proteins. AgrC can phosphorylate AgrA protein. The phosphorylated AgrA protein dimerizes and binds to the P2 promoter of the agrBDCA operon to form a positive feedback loop. The activity of AgrA dimers will also combine with P3 promoter, to activate the non-coding RNA, the RNA III expression. RNA III can affect the virulence factor and adhesion factor of the translation process.

Figure 1. Diagram of the Agr system of Staphylococcus aureus


We verified the expression capability by connecting sfGFP gene downstream (链接).

Design

Design Notes

We added some synonymous mutations to avoid part rules.

Source

We found this sequence data in the reported EMBL/GenBank/DDBJ Nucleotide Sequence Database.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]