Difference between revisions of "Part:BBa K3629001"

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===Usage and Biology===
 
===Usage and Biology===
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This is the natural promoter found in the wild-type <i>Yarrowia lipolytica</i> for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in <i>Yarrowia lipolytica.</i> Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels. It can be placed immediately upstream of a coding sequence for Y. lipolytica.
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This is the natural promoter found in the wild-type <i>Yarrowia lipolytica</i> for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in <i>Yarrowia lipolytica.</i> Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels (1). It can be placed immediately upstream of a coding sequence for Y. lipolytica.
  
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===Design===
 
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hellllloooooo where is this showing up
 
  
 
===Sequence and Features===
 
===Sequence and Features===
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===References===
 
===References===
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1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11
  
 
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Revision as of 06:13, 26 October 2020


Yarrowia lipolytica TEF1 promoter with an intron

Promoter from the Yarrowia lipolytica Translation Elongation Factor 1 (TEF1) gene with the first intron.

Usage and Biology

This is the natural promoter found in the wild-type Yarrowia lipolytica for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in Yarrowia lipolytica. Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels (1). It can be placed immediately upstream of a coding sequence for Y. lipolytica.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11

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