Difference between revisions of "Part:BBa K3365054:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | We get double terminator from plasmid pE1a-RFP kindly offered by team Tongji_China and get eGFP from plasmid HGT. | ||
===Source=== | ===Source=== | ||
| − | + | Due to complex secondary structure of double terminator, it is quite hard to add double terminator with eGFP fragment only by performing PCR twice. | |
===References=== | ===References=== | ||
Revision as of 06:11, 26 October 2020
enhanced GFP with double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 721
Illegal XhoI site found at 730 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We get double terminator from plasmid pE1a-RFP kindly offered by team Tongji_China and get eGFP from plasmid HGT.
Source
Due to complex secondary structure of double terminator, it is quite hard to add double terminator with eGFP fragment only by performing PCR twice.