Difference between revisions of "Part:BBa K3598022"
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This part was improved from Part BBa_K3089006--[[Part:BBa_K3089006|mTyr-CNK tyrosinase]]
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This part was improved from part BBa_K3089006--[[Part:BBa_K3089006|mTyr-CNK tyrosinase]]
  
 
This is the RBS lib construction system for mTyr-CNK expression. The T7 promoter is regulated by lacI repressor through lacO operator to control the expression of mTyr-CNK. The system is used to produce mTyr-CNK, so we construct a RBS library to improve the yield of mTyr-CNK.
 
This is the RBS lib construction system for mTyr-CNK expression. The T7 promoter is regulated by lacI repressor through lacO operator to control the expression of mTyr-CNK. The system is used to produce mTyr-CNK, so we construct a RBS library to improve the yield of mTyr-CNK.
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We began with randomly arranging six pairs in the system's RBS sequence TAAGTATAAGNNNNNNATAT, and verifying the resulting RBSs' strengths with RBS calculator. 15 of the strongest RBSs from verification are taken into our RBS library.  
 
We began with randomly arranging six pairs in the system's RBS sequence TAAGTATAAGNNNNNNATAT, and verifying the resulting RBSs' strengths with RBS calculator. 15 of the strongest RBSs from verification are taken into our RBS library.  
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[[File:T--BEIJING_4ELEVEN--Improvement_Figure_1.png|600px|thumb|center|Figure 1. Overall design of our RBS library]]
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Revision as of 05:18, 26 October 2020


LacI_LacI promoter_T7 promoter_lac operator_RBS lib_mTyr-CNK_T7 terminator


This part was improved from part BBa_K3089006--mTyr-CNK tyrosinase

This is the RBS lib construction system for mTyr-CNK expression. The T7 promoter is regulated by lacI repressor through lacO operator to control the expression of mTyr-CNK. The system is used to produce mTyr-CNK, so we construct a RBS library to improve the yield of mTyr-CNK.


Experiments and Results

We have improved the previous part BBa_K3089006 by using a series of new RBSs that differ in their expression efficiencies in expressing tyrosinase mTyr-CNK.

We began with randomly arranging six pairs in the system's RBS sequence TAAGTATAAGNNNNNNATAT, and verifying the resulting RBSs' strengths with RBS calculator. 15 of the strongest RBSs from verification are taken into our RBS library.

Figure 1. Overall design of our RBS library


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1280
    Illegal BsaI.rc site found at 1270