Difference between revisions of "Part:BBa K3629021"
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===References===
 
===References===
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[online] https://www.fpbase.org/protein/mcherry/ (Accessed October 26, 2020)  
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1. [online] https://www.fpbase.org/protein/mcherry/ (Accessed October 26, 2020)  
  
 
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Revision as of 05:00, 26 October 2020


mCherry codon optimized for Yarrowia lipolytica

mCherry coding sequence codon-optimized for Yarrowia lipolytica.

Usage and Biology

mCherry is a monomeric red fluorescent protein that is derived from DsRed of Discosoma. mCherry's optimal excitation wavelength is 586nm and its optimal emission wavelength is 610nm (1). mCherry could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy.

See BBa_K3629025 for a functional high expression construct using this coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1. [online] https://www.fpbase.org/protein/mcherry/ (Accessed October 26, 2020)