Difference between revisions of "Part:BBa K3629020"

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===Usage and Biology===
 
===Usage and Biology===
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Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a tryptophan binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain.
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Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a tryptophan binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain.
  
 
See [https://parts.igem.org/Part:BBa_K3629024 BBa_K3629024] for a high expression construct using this coding sequence.
 
See [https://parts.igem.org/Part:BBa_K3629024 BBa_K3629024] for a high expression construct using this coding sequence.

Revision as of 04:56, 26 October 2020


Yarrowia lipolytica TRP2 Gene

Anthranilate synthase component 1 (TRP2) coding sequence Yarrowia lipolytica .

Usage and Biology

Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a tryptophan binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain.

See BBa_K3629024 for a high expression construct using this coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 943
    Illegal NgoMIV site found at 1213
    Illegal NgoMIV site found at 1294
    Illegal NgoMIV site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 283
    Illegal BsaI.rc site found at 544
    Illegal BsaI.rc site found at 1441

References

1. [online] https://www.genome.jp/kegg-bin/show_pathway?yli00400 (Accessed October 26, 2020)