Difference between revisions of "Part:BBa K3593011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | add antigen tag that is more specific than 6*His tag | + | you may add antigen tag that is more specific than 6*His tag. |
| − | + | you may mutate this gene to reach a higher specificity. | |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 04:47, 26 October 2020
Single-Chain Fragment Variable (anti α-amatinin)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 98
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
you may add antigen tag that is more specific than 6*His tag. you may mutate this gene to reach a higher specificity.
Source
Artificially synthesized
References
1. Zhang X, He K, Zhao R, Feng T, Wei D. Development of a Single Chain Variable Fragment Antibody and Application as Amatoxin Recognition Molecule in Surface Plasmon Resonance Sensors. Food Anal Methods. 2016. doi:10.1007/s12161-016-0509-3
2. SAbPred: ABodyBuilder http://opig.stats.ox.ac.uk/webapps/newsabdab/sabpred/abodybuilder/