Difference between revisions of "Part:BBa K3624001"
Emily Hagens (Talk | contribs) |
|||
Line 3: | Line 3: | ||
<partinfo>BBa_K3624001 short</partinfo> | <partinfo>BBa_K3624001 short</partinfo> | ||
− | BMAP-18 is 18 amino acids long. It is the truncated version of the | + | BMAP-18 is effective against P. aeruginosa, S. maltophilia and trypanosomatid parasites. It is 18 amino acids long. It is the truncated version of the antimicrobial peptide BMAP-27, which is 27 amino acids long. BMAP-18 was used instead of BMAP-27 because it has increased target specificity and increased efficiency. This composite part includes the Ptac-M3 promoter (BBa_K3254017), RBS BBa_B0034, BMAP-18 coding region BBa_K3624000, and double terminator BBa_B0015. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 04:45, 26 October 2020
BMAP-18: Antimicrobial peptide.
BMAP-18 is effective against P. aeruginosa, S. maltophilia and trypanosomatid parasites. It is 18 amino acids long. It is the truncated version of the antimicrobial peptide BMAP-27, which is 27 amino acids long. BMAP-18 was used instead of BMAP-27 because it has increased target specificity and increased efficiency. This composite part includes the Ptac-M3 promoter (BBa_K3254017), RBS BBa_B0034, BMAP-18 coding region BBa_K3624000, and double terminator BBa_B0015.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 85
Illegal XbaI site found at 100
Illegal SpeI site found at 166
Illegal PstI site found at 180 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 85
Illegal SpeI site found at 166
Illegal PstI site found at 180
Illegal NotI site found at 91
Illegal NotI site found at 173 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 85
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 85
Illegal suffix found in sequence at 166 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 85
Illegal XbaI site found at 100
Illegal SpeI site found at 166
Illegal PstI site found at 180 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 144