Difference between revisions of "Part:BBa K3431031"

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	=== Introduction ===		=== Introduction ===
−	This superfolder green fluorescence protein (sfGFP) is derived from a modular synthetic biology education kit, banana toehold sensor sfGFP (Biobits) 1 , which can detect the RNA of banana and express the sfGFP.	+	This superfolder green fluorescence protein (sfGFP) is derived from a modular synthetic biology education kit, banana toehold sensor sfGFP (Biobits) <sup>1</sup> , which can detect the RNA of banana and express the sfGFP.
	===Characterization with banana toehold switch===		===Characterization with banana toehold switch===

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Revision as of 04:24, 26 October 2020

sfGFP

Introduction

This superfolder green fluorescence protein (sfGFP) is derived from a modular synthetic biology education kit, banana toehold sensor sfGFP (Biobits) ¹ , which can detect the RNA of banana and express the sfGFP.

Characterization with banana toehold switch

The 2020 iGEM CSMU_Taiwan characterized the sfGFP protein with the banana toehold sensor sfGFP (BBa_K3431050). We measured the real-time fluorescence within the 80 minutes protein production process.

Figure 1. The relative fluorescence of the sfGFP in the 80 minutes protein production process. The relative fluorescence was measured by the Synergy H1 Hybrid Multi-Mode Reader. The green line refers to the conditions with banana triggers, while the blue line refers to the conditions without a banana trigger.

Results As seen, the sfGFP generated a strong fluorescence signal, which makes it a great reporter for genetic devices.

Reference

1. Huang, A., Nguyen, P. Q., Stark, J. C., Takahashi, M. K., Donghia, N., Ferrante, T., Dy, A. J., Hsu, K. J., Dubner, R. S., Pardee, K., Jewett, M. C., & Collins, J. J. (2018). BioBits™ Explorer: A modular synthetic biology education kit. Science advances, 4(8), eaat5105. https://doi.org/10.1126/sciadv.aat5105 Sequence and Features