Difference between revisions of "Part:BBa K3552019"

 
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HofC is an inner membrane protein, a base for the generator of pilA.  This part is in the part collection where we have 12 genes that code for the base generator of pilA.  
 
HofC is an inner membrane protein, a base for the generator of pilA.  This part is in the part collection where we have 12 genes that code for the base generator of pilA.  
  
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The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552000 BBa_K3552001 BBa_K3552002. Parts that are the generator of the type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029. Parts that are a complete circuit: BBa_K3552009 BBa_K3552010 BBa_K3552011 BBa_K3552012.
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The part collection includes:
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Parts that are different kinds of type 4 pilus:
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<partinfo>BBa_K3552003</partinfo> 
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<partinfo>BBa_K3552004</partinfo>
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<partinfo>BBa_K3552005</partinfo>
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<partinfo>BBa_K3552006</partinfo>
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<partinfo>BBa_K3552007</partinfo>
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<partinfo>BBa_K3552008</partinfo>
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<partinfo>BBa_K3552018</partinfo> 
 +
<partinfo>BBa_K3552019</partinfo> 
 +
<partinfo>BBa_K3552020</partinfo> 
 +
<partinfo>BBa_K3552021</partinfo> 
 +
<partinfo>BBa_K3552022</partinfo> 
 +
<partinfo>BBa_K3552023</partinfo> 
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<partinfo>BBa_K3552024</partinfo> 
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<partinfo>BBa_K3552025</partinfo> 
 +
<partinfo>BBa_K3552026</partinfo> 
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<partinfo>BBa_K3552027</partinfo> 
 +
<partinfo>BBa_K3552028</partinfo> 
 +
<partinfo>BBa_K3552029</partinfo> 
  
 
Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.
 
Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.
  
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Usage and Biology:
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<span class='h3bb'>'''Sequence and Features'''</span>
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<partinfo>BBa_K3552019 SequenceAndFeatures</partinfo>
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==Usage and Biology==
 
The hofC is from the hof operon encoding the generator of major pilin, the ATPase HofB and the platform protein HofC is localized at 3 min of E.coli map. The HofB gene is a ATPase motor which localized at the base of the pilin generator system in the inner membrane, propel the assembly of pilus and it interacts with the platform protein HofC and HofM component of the inner membrane anchored assembly platform complex that also includes HofN and HofO. The  hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ.
 
The hofC is from the hof operon encoding the generator of major pilin, the ATPase HofB and the platform protein HofC is localized at 3 min of E.coli map. The HofB gene is a ATPase motor which localized at the base of the pilin generator system in the inner membrane, propel the assembly of pilus and it interacts with the platform protein HofC and HofM component of the inner membrane anchored assembly platform complex that also includes HofN and HofO. The  hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ.
  
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Characterization:
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==Characterization==
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We tried to uncoupled all the genes into single ones and we already separated them. We mutated the sequence and codon optimization of the repeated sequence from two genes to prevent recombination. In the future we are going to link all single genes to the same promotor and reconstructed all genes into a complete circuit to see any difference comparing to the initial combined genes.
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We tried to decoupled all the genes into single ones and we already separated them. We mutated the sequence and codon optimization of the repeated sequence from two genes to prevent recombination. In the future we are going to link all single genes to the same promotor and reconstructed all genes into a complete circuit to see any difference comparing to the initial combined genes.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3552019 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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<partinfo>BBa_K3552000 parameters</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3552019 parameters</partinfo>
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<!-- -->
 
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Revision as of 04:15, 26 October 2020


hofC

HofC is an inner membrane protein, a base for the generator of pilA. This part is in the part collection where we have 12 genes that code for the base generator of pilA.

The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029

Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 403
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The hofC is from the hof operon encoding the generator of major pilin, the ATPase HofB and the platform protein HofC is localized at 3 min of E.coli map. The HofB gene is a ATPase motor which localized at the base of the pilin generator system in the inner membrane, propel the assembly of pilus and it interacts with the platform protein HofC and HofM component of the inner membrane anchored assembly platform complex that also includes HofN and HofO. The hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ.

Characterization

We tried to decoupled all the genes into single ones and we already separated them. We mutated the sequence and codon optimization of the repeated sequence from two genes to prevent recombination. In the future we are going to link all single genes to the same promotor and reconstructed all genes into a complete circuit to see any difference comparing to the initial combined genes.