Difference between revisions of "Part:BBa K3552007"

 
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<partinfo>BBa_K3552007 short</partinfo>
 
<partinfo>BBa_K3552007 short</partinfo>
  
 
HofM is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofN is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization, DNA catabolic process, type IV pilus biogenesis and type IV pilus-dependent motility. HofO is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofP is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofQ is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Could be the porin responsible for transport of DNA across the outer membrane. Its functions include the carbon utilization, DNA binding, protein secretion and DNA catabolic process.This part is in the part collection where we have 13 genes that code for the proteins.  
 
HofM is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofN is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization, DNA catabolic process, type IV pilus biogenesis and type IV pilus-dependent motility. HofO is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofP is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofQ is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Could be the porin responsible for transport of DNA across the outer membrane. Its functions include the carbon utilization, DNA binding, protein secretion and DNA catabolic process.This part is in the part collection where we have 13 genes that code for the proteins.  
  
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HofM is a component of the IM complex that is connected to the outer membrane secretin channel formed by the hofQ and allows pilus exposure on the bacterial surface. HofN is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. HofO is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. The inner membrane anchored assembly platform complex is connected via hofP to the outer membrane secretin channel formed by hofQ that allows pilus to expose on the bacterial surface. The hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ. The hofMNOPQ is the generation pathway of pilA from the inner membrane to the surface of outer membrane.
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The part collection includes:
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Parts that are different kinds of type 4 pilus:
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<partinfo>BBa_K3552003</partinfo> 
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<partinfo>BBa_K3552004</partinfo>
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<partinfo>BBa_K3552005</partinfo>
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<partinfo>BBa_K3552006</partinfo>
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<partinfo>BBa_K3552007</partinfo>
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<partinfo>BBa_K3552008</partinfo>
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<partinfo>BBa_K3552018</partinfo> 
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<partinfo>BBa_K3552019</partinfo> 
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<partinfo>BBa_K3552020</partinfo> 
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<partinfo>BBa_K3552021</partinfo> 
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<partinfo>BBa_K3552022</partinfo> 
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<partinfo>BBa_K3552023</partinfo> 
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<partinfo>BBa_K3552024</partinfo> 
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<partinfo>BBa_K3552025</partinfo> 
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<partinfo>BBa_K3552026</partinfo> 
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<partinfo>BBa_K3552027</partinfo> 
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<partinfo>BBa_K3552028</partinfo> 
 +
<partinfo>BBa_K3552029</partinfo> 
  
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<!-- Add more about the biology of this part here
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Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.
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===Usage and Biology===
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<span class='h3bb'>'''Sequence and Features'''</span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3552000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3552007 SequenceAndFeatures</partinfo>
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==Usage and Biology==
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HofM is a component of the IM complex that is connected to the outer membrane secretin channel formed by the hofQ and allows pilus exposure on the bacterial surface. HofN is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. HofO is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. The inner membrane anchored assembly platform complex is connected via hofP to the outer membrane secretin channel formed by hofQ that allows pilus to expose on the bacterial surface. The hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ. The hofMNOPQ is the generation pathway of pilA from the inner membrane to the surface of outer membrane.
  
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==Characterization==
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We first uses the bacteria DH5a as the template and obtain the DNA fragment of 12 genes. Point mutations are carried out to alter the sequence into the same as the EHEC genes. The ygdB gene is reconstructed through the SOE pcr as it is so different from the required sequence. We verified the practicability of the generator through the western blot of pilA in vivo which confirmed the usage of the generator because the PilA is able to display outside the cell.
  
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<partinfo>BBa_K3552000 parameters</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3552007 parameters</partinfo>
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Revision as of 03:49, 26 October 2020


hofM-hofN-hofO-hofP-hofQ

HofM is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofN is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization, DNA catabolic process, type IV pilus biogenesis and type IV pilus-dependent motility. HofO is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofP is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofQ is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Could be the porin responsible for transport of DNA across the outer membrane. Its functions include the carbon utilization, DNA binding, protein secretion and DNA catabolic process.This part is in the part collection where we have 13 genes that code for the proteins.

The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029

Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

HofM is a component of the IM complex that is connected to the outer membrane secretin channel formed by the hofQ and allows pilus exposure on the bacterial surface. HofN is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. HofO is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. The inner membrane anchored assembly platform complex is connected via hofP to the outer membrane secretin channel formed by hofQ that allows pilus to expose on the bacterial surface. The hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ. The hofMNOPQ is the generation pathway of pilA from the inner membrane to the surface of outer membrane.

Characterization

We first uses the bacteria DH5a as the template and obtain the DNA fragment of 12 genes. Point mutations are carried out to alter the sequence into the same as the EHEC genes. The ygdB gene is reconstructed through the SOE pcr as it is so different from the required sequence. We verified the practicability of the generator through the western blot of pilA in vivo which confirmed the usage of the generator because the PilA is able to display outside the cell.