Difference between revisions of "Part:BBa K3573002:Experience"
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===Applications of BBa_K3573002=== | ===Applications of BBa_K3573002=== | ||
| + | Our team has amplified HRAS using PCR and cloned the gene into an expression vector. | ||
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| + | [[File:T--Korea_HS--RasPCR.jpg|500px]] | ||
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| + | We have tested protein expression in 4 different conditions (2 temperatures X 2 IPTG concentrations). After expression, Ni-NTA column was used for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS). | ||
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| + | [[File:T--Korea_HS--RasNiNTA.jpg|800px]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 00:03, 26 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3573002
Our team has amplified HRAS using PCR and cloned the gene into an expression vector.
We have tested protein expression in 4 different conditions (2 temperatures X 2 IPTG concentrations). After expression, Ni-NTA column was used for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS).
User Reviews
UNIQd3650242f3e730f8-partinfo-00000000-QINU UNIQd3650242f3e730f8-partinfo-00000001-QINU