Difference between revisions of "Part:BBa K3338007"

 
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The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-&#954;B/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated NF-&#954;B binding sites follwed by randomly generated AP-1 binding sites are localized.
 
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===Usage and Biology===
 
===Usage and Biology===
  
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The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-&#954;B/AP1 pathway leading to strong induction of gene expression. Due to its high basal activity but relatively low induction following LPS supplementation (as compared with <html><a href="https://parts.igem.org/Part:BBa_K3338002">BBa_K3338002</a></html>) it is perfectly suitable for application that need high promoter activities like rekombinant protein expression. Thhis synthetic promoter was designed as an improvement of the CMV promoter. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated NF-&#954;B binding sites follwed by randomly generated AP-1 binding sites are localized.
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
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<partinfo>BBa_K3338007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3338007 SequenceAndFeatures</partinfo>
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Revision as of 23:56, 25 October 2020


Synthetic promoter_1 with NF-κB and AP1 binding sites

Usage and Biology

The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-κB/AP1 pathway leading to strong induction of gene expression. Due to its high basal activity but relatively low induction following LPS supplementation (as compared with BBa_K3338002) it is perfectly suitable for application that need high promoter activities like rekombinant protein expression. Thhis synthetic promoter was designed as an improvement of the CMV promoter. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated NF-κB binding sites follwed by randomly generated AP-1 binding sites are localized.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NotI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]