Difference between revisions of "Part:BBa K3657053"
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<p>Lambda N binds to the BoxB RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). In that way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in proximity of a promoter, this construct will result in repression of the gene of interest.</p> | <p>Lambda N binds to the BoxB RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). In that way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in proximity of a promoter, this construct will result in repression of the gene of interest.</p> | ||
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| + | <p>More information here: LINK</p> | ||
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Revision as of 23:40, 25 October 2020
Lambda N - KRAB fusion protein
This part is a fusion protein consisting of a viral antiterminal protein lambda N (BBa_K3657014) and a KRAB repressor protein (BBa_K2549055).
Lambda N binds to the BoxB RNA hairpin (BBa_K3657015). In that way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in proximity of a promoter, this construct will result in repression of the gene of interest.
More information here: LINK
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]