Difference between revisions of "Part:BBa K3338002"

 
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<partinfo>BBa_K3338002 short</partinfo>
 
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The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-&#954;B/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated AP1-NF-&#954;B binding sites are localized.
 
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===Usage and Biology===
 
===Usage and Biology===
  
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The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-&#954;B/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. It was designed as an improvement of the CMV promoter making it LPS-sensing. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated AP1-NF-&#954;B binding sites are localized.
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
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<partinfo>BBa_K3338002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3338002 SequenceAndFeatures</partinfo>
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Revision as of 23:23, 25 October 2020


Synthetic promoter_2 with NF-κB and AP1 binding sites

Usage and Biology

The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-κB/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. It was designed as an improvement of the CMV promoter making it LPS-sensing. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated AP1-NF-κB binding sites are localized.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NotI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]