Difference between revisions of "Part:BBa K3484006"
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The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time. | The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time. | ||
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| + | [[File:UPF_Barcelona--Improve1.png|thumb|none|Figure 1. Experimental data extracted from the Plate-reader. Each curve is a mean of 4 different wells to reduce noise.]] | ||
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Revision as of 22:45, 25 October 2020
sfGFP + ASV tag
The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time.
| Table 1. Plate-Reader Parameters for the characterization of the effects of ASV tag in sfGFP | |||
| Parameters | Value | ||
| Plate-Reader model | Synergy HTX | ||
| Plate type | Thermo Fischer 96-well microplates black-walled clear bottom | ||
| Cell medium | LB | ||
| Time | 24 hours | ||
| Shake | Linear: Continuous, Frequency: 567 rpm (3mm) | ||
| Temperature | 37C | ||
| Gain | 50 | ||
| Optical Density (OD) measurement (absorbance) | 660nm | ||
| GFP excitation wavelength | 485nm | ||
| GFP emission wavelength | 528nm | ||
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 25
Illegal AgeI site found at 148 - 1000COMPATIBLE WITH RFC[1000]