Difference between revisions of "Part:BBa K3484006"

 
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The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time.
 
The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time.
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 +
{|class='wikitable'
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|colspan=4|Table 1. Parameters used to obtain the spectra
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|-
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|'''Parameters'''
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|'''Value'''
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|-
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|Plate-Reader model
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|Synergy HTX
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|-
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|Plate type
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|Thermo Fischer 96-well microplates black-walled clear bottom
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|-
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|Cell medium
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|LB
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|-
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|Time
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|24 hours
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|-
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|Shake
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|Linear: Continuous, Frequency: 567 rpm (3mm)
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|-
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|Temperature
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|37C
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|-
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|Gain
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|50
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|-
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|Optical Density (OD) measurement (absorbance)
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|660nm
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|-
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|GFP excitation wavelength
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|485nm
 +
|-
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|GFP emission wavelength
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|528nm
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|-
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|}
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:33, 25 October 2020


sfGFP + ASV tag

The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time.

Table 1. Parameters used to obtain the spectra
Parameters Value
Plate-Reader model Synergy HTX
Plate type Thermo Fischer 96-well microplates black-walled clear bottom
Cell medium LB
Time 24 hours
Shake Linear: Continuous, Frequency: 567 rpm (3mm)
Temperature 37C
Gain 50
Optical Density (OD) measurement (absorbance) 660nm
GFP excitation wavelength 485nm
GFP emission wavelength 528nm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]