Difference between revisions of "Part:BBa K3484006"
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The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time. | The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time. | ||
+ | |||
+ | {|class='wikitable' | ||
+ | |colspan=4|Table 1. Parameters used to obtain the spectra | ||
+ | |- | ||
+ | |'''Parameters''' | ||
+ | |'''Value''' | ||
+ | |- | ||
+ | |Plate-Reader model | ||
+ | |Synergy HTX | ||
+ | |- | ||
+ | |Plate type | ||
+ | |Thermo Fischer 96-well microplates black-walled clear bottom | ||
+ | |- | ||
+ | |Cell medium | ||
+ | |LB | ||
+ | |- | ||
+ | |Time | ||
+ | |24 hours | ||
+ | |- | ||
+ | |Shake | ||
+ | |Linear: Continuous, Frequency: 567 rpm (3mm) | ||
+ | |- | ||
+ | |Temperature | ||
+ | |37C | ||
+ | |- | ||
+ | |Gain | ||
+ | |50 | ||
+ | |- | ||
+ | |Optical Density (OD) measurement (absorbance) | ||
+ | |660nm | ||
+ | |- | ||
+ | |GFP excitation wavelength | ||
+ | |485nm | ||
+ | |- | ||
+ | |GFP emission wavelength | ||
+ | |528nm | ||
+ | |- | ||
+ | |} | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 22:33, 25 October 2020
sfGFP + ASV tag
The superfolder green fluorescent protein (sfGFP) is a variant of the common green fluorescent protein, that folds faster, thus generating fluorescent in less time.
Table 1. Parameters used to obtain the spectra | |||
Parameters | Value | ||
Plate-Reader model | Synergy HTX | ||
Plate type | Thermo Fischer 96-well microplates black-walled clear bottom | ||
Cell medium | LB | ||
Time | 24 hours | ||
Shake | Linear: Continuous, Frequency: 567 rpm (3mm) | ||
Temperature | 37C | ||
Gain | 50 | ||
Optical Density (OD) measurement (absorbance) | 660nm | ||
GFP excitation wavelength | 485nm | ||
GFP emission wavelength | 528nm |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 25
Illegal AgeI site found at 148 - 1000COMPATIBLE WITH RFC[1000]