Difference between revisions of "Part:BBa K3562002"
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[[Image:pLasRV fig2.png|frame|'''Figure 2''' Overview of the LasRV cell-free biosensor [2]]] | [[Image:pLasRV fig2.png|frame|'''Figure 2''' Overview of the LasRV cell-free biosensor [2]]] | ||
[[Image:pLasRV fig3.png|frame|'''Figure 3''' Detection of sputum sample using the LasRV cell-free biosensor [2]]] | [[Image:pLasRV fig3.png|frame|'''Figure 3''' Detection of sputum sample using the LasRV cell-free biosensor [2]]] | ||
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===Reference=== | ===Reference=== | ||
[1]Chappel J et al., Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41 (2013): 3471–3481. | [1]Chappel J et al., Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41 (2013): 3471–3481. | ||
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[2]Wen KY et al., A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples. ACS Synth. Biol., 6 (2017): 2293-2301. | [2]Wen KY et al., A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples. ACS Synth. Biol., 6 (2017): 2293-2301. |
Revision as of 21:03, 25 October 2020
pLasRV
las promoter from Pseudomonas aeruginosa
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 14
Usage and Biology
We found this well-characterized promoter pLasRV from P. aeruginosa cloned by Paul Freemont group in Imperial College, with in vivo and in vitro expression data thoroughly presented in the research articles [1]. Notably, researchers developed a cell-free biosensor based on this promoter to detect the concentration of OdDHL in sputum samples [2]. More attempts to work on this inducible promoter in iGEM will deepen the understanding of quorum sensing in P. aeruginosa and facilitate the exploitation of biomarker-based biosensors.
Reference
[1]Chappel J et al., Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41 (2013): 3471–3481.
[2]Wen KY et al., A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples. ACS Synth. Biol., 6 (2017): 2293-2301.